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A time-resolved fluorescence resonance energy transfer screening assay for discovery of protein-protein interaction modulators

Protein-protein interactions (PPIs) have emerged as promising yet challenging therapeutic targets. A robust bioassay is required for rapid PPI modulator discovery. Here, we present a time-resolved Förster's (fluorescence) resonance energy transfer assay protocol for PPI modulator screening in a...

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Detalles Bibliográficos
Autores principales: Tang, Cong, Niu, Qiankun, Cicka, Danielle, Du, Yuhong, Mo, Xiulei, Fu, Haian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433285/
https://www.ncbi.nlm.nih.gov/pubmed/34527960
http://dx.doi.org/10.1016/j.xpro.2021.100804