Combining protein and RNA quantification to evaluate promoter activity by using dual-color fluorescent reporting systems

Herein, Broccoli/mCherry and an EGFP/mCherry dual-color fluorescent reporting systems have been established to quantify the promoter activity at transcription and translation levels in eukaryotic cells. Based on those systems, four commonly used promoters (CMV and SV40 of Pol II and U6, H1 of Pol II...

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Detalles Bibliográficos
Autores principales: Peng, Yan, Huang, Xin, Huang, Tianfang, Du, Feng, Cui, Xin, Tang, Zhuo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Portland Press Ltd. 2021
Materias:
Biochemical Techniques & Resources
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433482/
https://www.ncbi.nlm.nih.gov/pubmed/34467968
http://dx.doi.org/10.1042/BSR20211525

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8433482/
https://www.ncbi.nlm.nih.gov/pubmed/34467968
http://dx.doi.org/10.1042/BSR20211525

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