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Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease
INTRODUCTION: The substantial link between apolipoprotein E (APOE) ε4 allele and oxidative stress may underlie enhanced Alzheimer's disease (AD) risk. Here, we studied the impact of APOE ε4 on the level of apolipoproteins with antioxidant activities along with oxidative markers in circulating e...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438681/ https://www.ncbi.nlm.nih.gov/pubmed/34541286 http://dx.doi.org/10.1002/dad2.12231 |
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author | Ben Khedher, Mohamed Raâfet Haddad, Mohamed Laurin, Danielle Ramassamy, Charles |
author_facet | Ben Khedher, Mohamed Raâfet Haddad, Mohamed Laurin, Danielle Ramassamy, Charles |
author_sort | Ben Khedher, Mohamed Raâfet |
collection | PubMed |
description | INTRODUCTION: The substantial link between apolipoprotein E (APOE) ε4 allele and oxidative stress may underlie enhanced Alzheimer's disease (AD) risk. Here, we studied the impact of APOE ε4 on the level of apolipoproteins with antioxidant activities along with oxidative markers in circulating extracellular vesicles (cEVs) and plasma from cognitively impaired‐not demented (CIND) individuals converted to AD (CIND‐AD). METHODS: Apolipoproteins E, J, and D and antioxidant response markers were determined in cEVs and plasma using immunoblotting, electrochemical examination, and spectrofluorimetry. RESULTS: Total antioxidant capacity and apolipoprotein D levels in cEVs, as judged by regression analysis and cognitive performance correlations, allowed us to differentiate CIND APOE ε4 carriers from controls and to predict their progression to AD 5 years later. DISCUSSION: Our findings support the pathological redox linkage between APOE ε4 and AD onset and suggest the use of cEVs oxidative signature in early AD diagnosis. |
format | Online Article Text |
id | pubmed-8438681 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-84386812021-09-17 Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease Ben Khedher, Mohamed Raâfet Haddad, Mohamed Laurin, Danielle Ramassamy, Charles Alzheimers Dement (Amst) Blood‐based Biomarkers INTRODUCTION: The substantial link between apolipoprotein E (APOE) ε4 allele and oxidative stress may underlie enhanced Alzheimer's disease (AD) risk. Here, we studied the impact of APOE ε4 on the level of apolipoproteins with antioxidant activities along with oxidative markers in circulating extracellular vesicles (cEVs) and plasma from cognitively impaired‐not demented (CIND) individuals converted to AD (CIND‐AD). METHODS: Apolipoproteins E, J, and D and antioxidant response markers were determined in cEVs and plasma using immunoblotting, electrochemical examination, and spectrofluorimetry. RESULTS: Total antioxidant capacity and apolipoprotein D levels in cEVs, as judged by regression analysis and cognitive performance correlations, allowed us to differentiate CIND APOE ε4 carriers from controls and to predict their progression to AD 5 years later. DISCUSSION: Our findings support the pathological redox linkage between APOE ε4 and AD onset and suggest the use of cEVs oxidative signature in early AD diagnosis. John Wiley and Sons Inc. 2021-09-14 /pmc/articles/PMC8438681/ /pubmed/34541286 http://dx.doi.org/10.1002/dad2.12231 Text en © 2021 The Authors. Alzheimer's & Dementia: Diagnosis, Assessment & Disease Monitoring published by Wiley Periodicals, LLC on behalf of Alzheimer's Association https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Blood‐based Biomarkers Ben Khedher, Mohamed Raâfet Haddad, Mohamed Laurin, Danielle Ramassamy, Charles Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease |
title | Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease |
title_full | Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease |
title_fullStr | Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease |
title_full_unstemmed | Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease |
title_short | Effect of APOE ε4 allele on levels of apolipoproteins E, J, and D, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to Alzheimer's disease |
title_sort | effect of apoe ε4 allele on levels of apolipoproteins e, j, and d, and redox signature in circulating extracellular vesicles from cognitively impaired with no dementia participants converted to alzheimer's disease |
topic | Blood‐based Biomarkers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8438681/ https://www.ncbi.nlm.nih.gov/pubmed/34541286 http://dx.doi.org/10.1002/dad2.12231 |
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