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Laboratory Exercise to Measure Plasmid Copy Number by qPCR

Quantitative PCR (qPCR) has numerous applications in biology. In an educational setting, qPCR provides students an opportunity to better understand the PCR mechanism by providing both quantitative information about the reactions and also data to troubleshoot PCRs (e.g., melt curves). Here, we presen...

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Detalles Bibliográficos
Autores principales: David, Benjamin, Li, Jinbei, Masood, Faisal, Blassick, Caroline, Jensen, Paul, Jensen, Karin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Microbiology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8442022/
https://www.ncbi.nlm.nih.gov/pubmed/34594455
http://dx.doi.org/10.1128/jmbe.00125-21