Single-molecule kinetic locking allows fluorescence-free quantification of protein/nucleic-acid binding

Fluorescence-free micro-manipulation of nucleic acids (NA) allows the functional characterization of DNA/RNA processing proteins, without the interference of labels, but currently fails to detect and quantify their binding. To overcome this limitation, we developed a method based on single-molecule...

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Detalles Bibliográficos
Autores principales: Rieu, Martin, Valle-Orero, Jessica, Ducos, Bertrand, Allemand, Jean-François, Croquette, Vincent
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443601/
https://www.ncbi.nlm.nih.gov/pubmed/34526657
http://dx.doi.org/10.1038/s42003-021-02606-z

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443601/
https://www.ncbi.nlm.nih.gov/pubmed/34526657
http://dx.doi.org/10.1038/s42003-021-02606-z

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