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EndoBind detects endogenous protein-protein interactions in real time

We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CR...

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Detalles Bibliográficos
Autores principales: Bill, Anke, Espinola, Sheryll, Guthy, Daniel, Haling, Jacob R., Lanter, Mylene, Lu, Min, Marelli, Anthony, Mendiola, Angelica, Miraglia, Loren, Taylor, Brandon L., Vargas, Leonardo, Orth, Anthony P., King, Frederick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443649/
https://www.ncbi.nlm.nih.gov/pubmed/34526658
http://dx.doi.org/10.1038/s42003-021-02600-5