Cargando…
EndoBind detects endogenous protein-protein interactions in real time
We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CR...
| Autores principales: | , , , , , , , , , , , , |
|---|---|
| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Nature Publishing Group UK
2021
|
| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443649/ https://www.ncbi.nlm.nih.gov/pubmed/34526658 http://dx.doi.org/10.1038/s42003-021-02600-5 |
| _version_ | 1784568327909146624 |
|---|---|
| author | Bill, Anke Espinola, Sheryll Guthy, Daniel Haling, Jacob R. Lanter, Mylene Lu, Min Marelli, Anthony Mendiola, Angelica Miraglia, Loren Taylor, Brandon L. Vargas, Leonardo Orth, Anthony P. King, Frederick J. |
| author_facet | Bill, Anke Espinola, Sheryll Guthy, Daniel Haling, Jacob R. Lanter, Mylene Lu, Min Marelli, Anthony Mendiola, Angelica Miraglia, Loren Taylor, Brandon L. Vargas, Leonardo Orth, Anthony P. King, Frederick J. |
| author_sort | Bill, Anke |
| collection | PubMed |
| description | We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time. |
| format | Online Article Text |
| id | pubmed-8443649 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2021 |
| publisher | Nature Publishing Group UK |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-84436492021-10-04 EndoBind detects endogenous protein-protein interactions in real time Bill, Anke Espinola, Sheryll Guthy, Daniel Haling, Jacob R. Lanter, Mylene Lu, Min Marelli, Anthony Mendiola, Angelica Miraglia, Loren Taylor, Brandon L. Vargas, Leonardo Orth, Anthony P. King, Frederick J. Commun Biol Article We present two high-throughput compatible methods to detect the interaction of ectopically expressed (RT-Bind) or endogenously tagged (EndoBind) proteins of interest. Both approaches provide temporal evaluation of dimer formation over an extended duration. Using examples of the Nrf2-KEAP1 and the CRAF-KRAS-G12V interaction, we demonstrate that our method allows for the detection of signal for more than 2 days after substrate addition, allowing for continuous monitoring of endogenous protein-protein interactions in real time. Nature Publishing Group UK 2021-09-15 /pmc/articles/PMC8443649/ /pubmed/34526658 http://dx.doi.org/10.1038/s42003-021-02600-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
| spellingShingle | Article Bill, Anke Espinola, Sheryll Guthy, Daniel Haling, Jacob R. Lanter, Mylene Lu, Min Marelli, Anthony Mendiola, Angelica Miraglia, Loren Taylor, Brandon L. Vargas, Leonardo Orth, Anthony P. King, Frederick J. EndoBind detects endogenous protein-protein interactions in real time |
| title | EndoBind detects endogenous protein-protein interactions in real time |
| title_full | EndoBind detects endogenous protein-protein interactions in real time |
| title_fullStr | EndoBind detects endogenous protein-protein interactions in real time |
| title_full_unstemmed | EndoBind detects endogenous protein-protein interactions in real time |
| title_short | EndoBind detects endogenous protein-protein interactions in real time |
| title_sort | endobind detects endogenous protein-protein interactions in real time |
| topic | Article |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8443649/ https://www.ncbi.nlm.nih.gov/pubmed/34526658 http://dx.doi.org/10.1038/s42003-021-02600-5 |
| work_keys_str_mv | AT billanke endobinddetectsendogenousproteinproteininteractionsinrealtime AT espinolasheryll endobinddetectsendogenousproteinproteininteractionsinrealtime AT guthydaniel endobinddetectsendogenousproteinproteininteractionsinrealtime AT halingjacobr endobinddetectsendogenousproteinproteininteractionsinrealtime AT lantermylene endobinddetectsendogenousproteinproteininteractionsinrealtime AT lumin endobinddetectsendogenousproteinproteininteractionsinrealtime AT marellianthony endobinddetectsendogenousproteinproteininteractionsinrealtime AT mendiolaangelica endobinddetectsendogenousproteinproteininteractionsinrealtime AT miraglialoren endobinddetectsendogenousproteinproteininteractionsinrealtime AT taylorbrandonl endobinddetectsendogenousproteinproteininteractionsinrealtime AT vargasleonardo endobinddetectsendogenousproteinproteininteractionsinrealtime AT orthanthonyp endobinddetectsendogenousproteinproteininteractionsinrealtime AT kingfrederickj endobinddetectsendogenousproteinproteininteractionsinrealtime |