Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy

Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular stud...

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Autores principales: Mann, Guy, Satish, Gandhesiri, Sulkshane, Prasad, Mandal, Shaswati, Glickman, Michael H., Brik, Ashraf
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2021
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445162/
https://www.ncbi.nlm.nih.gov/pubmed/34528945
http://dx.doi.org/10.1039/d1cc04045f
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author Mann, Guy
Satish, Gandhesiri
Sulkshane, Prasad
Mandal, Shaswati
Glickman, Michael H.
Brik, Ashraf
author_facet Mann, Guy
Satish, Gandhesiri
Sulkshane, Prasad
Mandal, Shaswati
Glickman, Michael H.
Brik, Ashraf
author_sort Mann, Guy
collection PubMed
description Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagy via direct visualization of a phosphorylated ubiquitin probe in the cellular environment.
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spelling pubmed-84451622021-10-18 Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy Mann, Guy Satish, Gandhesiri Sulkshane, Prasad Mandal, Shaswati Glickman, Michael H. Brik, Ashraf Chem Commun (Camb) Chemistry Protein post-translational modifications are involved in essentially all aspects of cellular signaling. Their dynamic nature and the difficulties in installing them using enzymatic approaches limits their direct study in human cells. Reported herein is the first synthesis, delivery and cellular study of a stable phosphoubiquitin probe. Our results compare Parkin's substrate preference during mitophagy via direct visualization of a phosphorylated ubiquitin probe in the cellular environment. The Royal Society of Chemistry 2021-08-26 /pmc/articles/PMC8445162/ /pubmed/34528945 http://dx.doi.org/10.1039/d1cc04045f Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/
spellingShingle Chemistry
Mann, Guy
Satish, Gandhesiri
Sulkshane, Prasad
Mandal, Shaswati
Glickman, Michael H.
Brik, Ashraf
Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
title Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
title_full Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
title_fullStr Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
title_full_unstemmed Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
title_short Synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
title_sort synthesis and delivery of a stable phosphorylated ubiquitin probe to study ubiquitin conjugation in mitophagy
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445162/
https://www.ncbi.nlm.nih.gov/pubmed/34528945
http://dx.doi.org/10.1039/d1cc04045f
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