Fast in vitro protocol for the visualization and quantitative high-throughput analysis of sprouting angiogenesis by confocal microscopy

We describe an optimized, cost-effective, reproducible, and robust protocol to study sprouting angiogenesis in glass-bottom 96-well plates by confocal microscopy, ideal for screening of drug or shRNA libraries. Effective and stable knockdown of gene expression in primary endothelial cells is achieve...

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Detalles Bibliográficos
Autores principales: Kempers, Lanette, van der Bijl, Ivo, van Stalborch, Anne-Marieke D., Ponsioen, Bas, Margadant, Coert
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445886/
https://www.ncbi.nlm.nih.gov/pubmed/34557696
http://dx.doi.org/10.1016/j.xpro.2021.100690

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445886/
https://www.ncbi.nlm.nih.gov/pubmed/34557696
http://dx.doi.org/10.1016/j.xpro.2021.100690

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