A bioluminescent and homogeneous SARS-CoV-2 spike RBD and hACE2 interaction assay for antiviral screening and monitoring patient neutralizing antibody levels

Here we describe a homogeneous bioluminescent immunoassay based on the interaction between Fc-tagged SARS-CoV-2 Spike RBD and human ACE2, and its detection by secondary antibodies labeled with NanoLuc luciferase fragments LgBit and SmBit. The assay utility for the discovery of novel inhibitors was d...

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Detalles Bibliográficos
Autores principales: Alves, Juliano, Engel, Laurie, de Vasconcelos Cabral, Renata, Rodrigues, Eduardo L., de Jesus Ribeiro, Liane, Higa, Luiza M., da Costa Ferreira Júnior, Orlando, Castiñeiras, Terezinha Marta P. P., de Carvalho Leitão, Isabela, Tanuri, Amilcar, Goueli, Said A., Zegzouti, Hicham
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445915/
https://www.ncbi.nlm.nih.gov/pubmed/34531417
http://dx.doi.org/10.1038/s41598-021-97330-3

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8445915/
https://www.ncbi.nlm.nih.gov/pubmed/34531417
http://dx.doi.org/10.1038/s41598-021-97330-3

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