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Improvements in Extraction Methods of High-molecular-weight DNA from Soils by Modifying Cell Lysis Conditions and Reducing Adsorption of DNA onto Soil Particles
High-molecular-weight DNA (HMW DNA) extracted from soil is useful for examined the functions and diversity of soil organisms, the majority of which are difficult to culture. In the present study, the procedures used to extract HMW DNA from soil samples were improved. The grinding of soil samples wit...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions / Japanese Society for Extremophiles
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8446751/ https://www.ncbi.nlm.nih.gov/pubmed/34234043 http://dx.doi.org/10.1264/jsme2.ME21017 |