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Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449058/ https://www.ncbi.nlm.nih.gov/pubmed/34568840 http://dx.doi.org/10.1016/j.xpro.2021.100813 |