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Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449058/ https://www.ncbi.nlm.nih.gov/pubmed/34568840 http://dx.doi.org/10.1016/j.xpro.2021.100813 |
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author | Junyent, Sergi Reeves, Joshua Habib, Shukry J. |
author_facet | Junyent, Sergi Reeves, Joshua Habib, Shukry J. |
author_sort | Junyent, Sergi |
collection | PubMed |
description | These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes cytoneme reactivity during Wnt-interaction initiation, Ca(2+) transients at Wnt-contacting cytonemes, and subsequent pairing between ESCs and Wnt-sources. This pairing interaction is crucial to synthetic embryogenesis; hence this protocol is effective for in vitro studies of developmental biology. For complete details on the use and execution of this protocol, please refer to Junyent et al. (2020, 2021a, 2021b). |
format | Online Article Text |
id | pubmed-8449058 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-84490582021-09-24 Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach Junyent, Sergi Reeves, Joshua Habib, Shukry J. STAR Protoc Protocol These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes cytoneme reactivity during Wnt-interaction initiation, Ca(2+) transients at Wnt-contacting cytonemes, and subsequent pairing between ESCs and Wnt-sources. This pairing interaction is crucial to synthetic embryogenesis; hence this protocol is effective for in vitro studies of developmental biology. For complete details on the use and execution of this protocol, please refer to Junyent et al. (2020, 2021a, 2021b). Elsevier 2021-09-15 /pmc/articles/PMC8449058/ /pubmed/34568840 http://dx.doi.org/10.1016/j.xpro.2021.100813 Text en © 2021 King's College London https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Protocol Junyent, Sergi Reeves, Joshua Habib, Shukry J. Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
title | Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
title_full | Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
title_fullStr | Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
title_full_unstemmed | Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
title_short | Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
title_sort | assessing the wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach |
topic | Protocol |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449058/ https://www.ncbi.nlm.nih.gov/pubmed/34568840 http://dx.doi.org/10.1016/j.xpro.2021.100813 |
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