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Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach

These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes...

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Detalles Bibliográficos
Autores principales: Junyent, Sergi, Reeves, Joshua, Habib, Shukry J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449058/
https://www.ncbi.nlm.nih.gov/pubmed/34568840
http://dx.doi.org/10.1016/j.xpro.2021.100813
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author Junyent, Sergi
Reeves, Joshua
Habib, Shukry J.
author_facet Junyent, Sergi
Reeves, Joshua
Habib, Shukry J.
author_sort Junyent, Sergi
collection PubMed
description These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes cytoneme reactivity during Wnt-interaction initiation, Ca(2+) transients at Wnt-contacting cytonemes, and subsequent pairing between ESCs and Wnt-sources. This pairing interaction is crucial to synthetic embryogenesis; hence this protocol is effective for in vitro studies of developmental biology. For complete details on the use and execution of this protocol, please refer to Junyent et al. (2020, 2021a, 2021b).
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spelling pubmed-84490582021-09-24 Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach Junyent, Sergi Reeves, Joshua Habib, Shukry J. STAR Protoc Protocol These protocols investigate the interaction of cytonemes with localized Wnt. Cell-niche signaling between naive or primed mouse embryonic stem cells (ESCs) and either Wnt-secreting trophoblast stem cells (TSCs) or Wnt signals tethered to microbeads can be scrutinized in vitro. This approach analyzes cytoneme reactivity during Wnt-interaction initiation, Ca(2+) transients at Wnt-contacting cytonemes, and subsequent pairing between ESCs and Wnt-sources. This pairing interaction is crucial to synthetic embryogenesis; hence this protocol is effective for in vitro studies of developmental biology. For complete details on the use and execution of this protocol, please refer to Junyent et al. (2020, 2021a, 2021b). Elsevier 2021-09-15 /pmc/articles/PMC8449058/ /pubmed/34568840 http://dx.doi.org/10.1016/j.xpro.2021.100813 Text en © 2021 King's College London https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Junyent, Sergi
Reeves, Joshua
Habib, Shukry J.
Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
title Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
title_full Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
title_fullStr Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
title_full_unstemmed Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
title_short Assessing the Wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
title_sort assessing the wnt-reactivity of cytonemes of mouse embryonic stem cells using a bioengineering approach
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449058/
https://www.ncbi.nlm.nih.gov/pubmed/34568840
http://dx.doi.org/10.1016/j.xpro.2021.100813
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