Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast

Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry. It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characteri...

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Detalles Bibliográficos
Autores principales: Challa, Kiran, Seebacher, Jan, Gasser, Susan M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449121/
https://www.ncbi.nlm.nih.gov/pubmed/34568845
http://dx.doi.org/10.1016/j.xpro.2021.100825

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449121/
https://www.ncbi.nlm.nih.gov/pubmed/34568845
http://dx.doi.org/10.1016/j.xpro.2021.100825

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