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Sucrose gradient chromatin enrichment for quantitative proteomics analysis in budding yeast

Here, we describe a fractionation protocol optimized to quantify changes in relative abundance of the chromatin-bound proteome (chromatome) by tandem mass tag multiplexing-based tandem mass spectrometry. It has been applied to yeast cells before and after exposure to DNA-damaging drugs to characteri...

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Detalles Bibliográficos
Autores principales:	Challa, Kiran, Seebacher, Jan, Gasser, Susan M.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449121/ https://www.ncbi.nlm.nih.gov/pubmed/34568845 http://dx.doi.org/10.1016/j.xpro.2021.100825

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