Improved CRISPR/Cas9 knock-in efficiency via the self-excising cassette (SEC) selection method in C. elegans

Streamlined, selection-based CRISPR knock-in protocols for C. elegans were first introduced six years ago (Dickinson et al. 2015; Schwartz and Jorgensen 2016). Though these selection-based approaches are powerful, one drawback has been the requirement to inject large numbers of P0 worms (~30-60 per...

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Detalles Bibliográficos
Autores principales: Huang, George, de Jesus, Bailey, Koh, Alex, Blanco, Sara, Rettmann, Aubrie, DeMott, Ella, Sylvester, Melynda, Ren, Cassie, Meng, Carrie, Waterland, Skye, Rhodes, Anita, Alicea, Persephone, Flynn, Abbey, Dickinson, Daniel J, Doonan, Ryan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2021
Materias:
New Finding
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449260/
https://www.ncbi.nlm.nih.gov/pubmed/34549176
http://dx.doi.org/10.17912/micropub.biology.000460

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8449260/
https://www.ncbi.nlm.nih.gov/pubmed/34549176
http://dx.doi.org/10.17912/micropub.biology.000460

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