A flow-cytometry-based assessment of global protein synthesis in human senescent cells

Senescent cells constantly experience stressful conditions and restrain their protein translation to cope with it. Here, we present a detailed protocol to measure the rate of global protein synthesis using L-azidohomoalanine (L-AHA)-based click chemistry in human senescent fibroblasts. We optimized...

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Detalles Bibliográficos
Autores principales: Lee, Yeonghyeon, Kim, Jaejin, Jeon, Taerang, Roh, Kyeonghwan, Kim, Mi-Sung, Kang, Chanhee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450257/
https://www.ncbi.nlm.nih.gov/pubmed/34585148
http://dx.doi.org/10.1016/j.xpro.2021.100809

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450257/
https://www.ncbi.nlm.nih.gov/pubmed/34585148
http://dx.doi.org/10.1016/j.xpro.2021.100809

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