Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo

Significant regulation of gene expression is mediated at the translation level. Here, we describe protocols for imaging and analysis of translation at single mRNA resolution in both fixed and living Drosophila embryos. These protocols use the SunTag system, in which the protein of interest is visual...

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Detalles Bibliográficos
Autores principales: Vinter, Daisy J., Hoppe, Caroline, Ashe, Hilary L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450298/
https://www.ncbi.nlm.nih.gov/pubmed/34585149
http://dx.doi.org/10.1016/j.xpro.2021.100812

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450298/
https://www.ncbi.nlm.nih.gov/pubmed/34585149
http://dx.doi.org/10.1016/j.xpro.2021.100812

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