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Live and fixed imaging of translation sites at single mRNA resolution in the Drosophila embryo
Significant regulation of gene expression is mediated at the translation level. Here, we describe protocols for imaging and analysis of translation at single mRNA resolution in both fixed and living Drosophila embryos. These protocols use the SunTag system, in which the protein of interest is visual...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8450298/ https://www.ncbi.nlm.nih.gov/pubmed/34585149 http://dx.doi.org/10.1016/j.xpro.2021.100812 |