Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins

The isolation of protein-RNA complexes in the “denaturing cross-linked RNA immunoprecipitation” (dCLIP) protocol is based on biotin-tagging proteins of interest, UV cross-linking RNA to protein in vivo, RNase protection assay, and isolating RNA-protein complexes under denaturing conditions over a st...

Descripción completa

Detalles Bibliográficos
Autores principales: Rosenberg, Michael, Levy, Vered, Maier, Verena K., Kesner, Barry, Blum, Roy, Lee, Jeannie T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452891/
https://www.ncbi.nlm.nih.gov/pubmed/34585157
http://dx.doi.org/10.1016/j.xpro.2021.100819

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452891/
https://www.ncbi.nlm.nih.gov/pubmed/34585157
http://dx.doi.org/10.1016/j.xpro.2021.100819

Ejemplares similares