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Denaturing cross-linking immunoprecipitation to identify footprints for RNA-binding proteins

The isolation of protein-RNA complexes in the “denaturing cross-linked RNA immunoprecipitation” (dCLIP) protocol is based on biotin-tagging proteins of interest, UV cross-linking RNA to protein in vivo, RNase protection assay, and isolating RNA-protein complexes under denaturing conditions over a st...

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Detalles Bibliográficos
Autores principales:	Rosenberg, Michael, Levy, Vered, Maier, Verena K., Kesner, Barry, Blum, Roy, Lee, Jeannie T.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8452891/ https://www.ncbi.nlm.nih.gov/pubmed/34585157 http://dx.doi.org/10.1016/j.xpro.2021.100819

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