Cargando…

Rigidifying a De Novo Enzyme Increases Activity and Induces a Negative Activation Heat Capacity

[Image: see text] Conformational sampling profoundly impacts the overall activity and temperature dependence of enzymes. Peroxidases have emerged as versatile platforms for high-value biocatalysis owing to their broad palette of potential biotransformations. Here, we explore the role of conformation...

Descripción completa

Detalles Bibliográficos
Autores principales:	Hindson, Sarah A., Bunzel, H. Adrian, Frank, Bettina, Svistunenko, Dimitri A., Williams, Christopher, van der Kamp, Marc W., Mulholland, Adrian J., Pudney, Christopher R., Anderson, J. L. Ross
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2021
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8453482/ https://www.ncbi.nlm.nih.gov/pubmed/34557328 http://dx.doi.org/10.1021/acscatal.1c01776

Ejemplares similares

Dynamical origins of heat capacity changes in enzyme-catalysed reactions
por: van der Kamp, Marc W., et al.
Publicado: (2018)

Sensing Enzyme Activation Heat Capacity at the Single-Molecule Level Using Gold-Nanorod-Based Optical Whispering Gallery Modes
por: Subramanian, Sivaraman, et al.
Publicado: (2021)

A Rigidifying Salt-Bridge Favors the Activity of Thermophilic Enzyme at High Temperatures at the Expense of Low-Temperature Activity
por: Lam, Sonia Y., et al.
Publicado: (2011)

Novel African Trypanocidal Agents: Membrane Rigidifying Peptides
por: Harrington, John M., et al.
Publicado: (2012)

High affinity rigidified AT(2) receptor ligands with indane scaffolds
por: Wallinder, Charlotta, et al.
Publicado: (2019)

QM/MM simulations identify the determinants of catalytic activity differences between type II dehydroquinase enzymes
por: Lence, Emilio, et al.
Publicado: (2018)

Synthesis of rigidified flavin–guanidinium ion conjugates and investigation of their photocatalytic properties
por: Schmaderer, Harald, et al.
Publicado: (2009)

T-Cell Receptor Variable β Domains Rigidify During Affinity Maturation
por: Fernández-Quintero, Monica L., et al.
Publicado: (2020)

[2]-Ladderanes as isosteres for meta-substituted aromatic rings and rigidified cyclohexanes
por: Epplin, Rachel C., et al.
Publicado: (2022)

Expanding the Functional Scope of the Fmoc‐Diphenylalanine Hydrogelator by Introducing a Rigidifying and Chemically Active Urea Backbone Modification
por: Basavalingappa, Vasantha, et al.
Publicado: (2019)

Rigidified and Hydrophilic DOTA-like Lanthanoid Ligands: Design, Synthesis, and Dynamic Properties
por: Miao, Qing, et al.
Publicado: (2023)

2,5-disubstituted bicyclo[2.1.1]hexanes as rigidified cyclopentane variants
por: Paul, Shashwati, et al.
Publicado: (2023)

An expandable, modular de novo protein platform for precision redox engineering
por: Hutchins, George H., et al.
Publicado: (2023)

Rigidified Derivative of the Non-macrocyclic Ligand H(4)OCTAPA for Stable Lanthanide(III) Complexation
por: Lucio-Martínez, Fátima, et al.
Publicado: (2022)

Construction and in vivo assembly of a catalytically proficient and hyperthermostable de novo enzyme
por: Watkins, Daniel W., et al.
Publicado: (2017)

Effect of the distal histidine on the peroxidatic activity of monomeric cytoglobin
por: Beckerson, Penny, et al.
Publicado: (2015)

Chemical Mapping Exposes the Importance of Active Site Interactions in Governing the Temperature Dependence of Enzyme Turnover
por: Winter, Samuel D., et al.
Publicado: (2021)

Shark Antibody Variable Domains Rigidify Upon Affinity Maturation—Understanding the Potential of Shark Immunoglobulins as Therapeutics
por: Fernández-Quintero, Monica L., et al.
Publicado: (2021)

NMR analysis suggests the terminal domains of Robo1 remain extended but are rigidified in the presence of heparan sulfate
por: Williams, Robert V., et al.
Publicado: (2022)

Structure and Function in Homodimeric Enzymes: Simulations of Cooperative and Independent Functional Motions
por: Wells, Stephen A., et al.
Publicado: (2015)

Organic room-temperature phosphorescence from halogen-bonded organic frameworks: hidden electronic effects in rigidified chromophores
por: Zhou, Jiawang, et al.
Publicado: (2020)

Rigidified Bis(sulfonyl)ethylenes as Effective Michael Acceptors for Asymmetric Catalysis: Application to the Enantioselective Synthesis of Quaternary Hydantoins
por: Villaescusa, Leire, et al.
Publicado: (2023)

Chemical accuracy in QM/MM calculations on enzyme-catalysed reactions
por: Mulholland, Adrian J
Publicado: (2007)

Programmably tiling rigidified DNA brick on gold nanoparticle as multi-functional shell for cancer-targeted delivery of siRNAs
por: Xue, Chang, et al.
Publicado: (2021)

Novel Helical Trp- and Arg-Rich Antimicrobial Peptides Locate Near Membrane Surfaces and Rigidify Lipid Model Membranes
por: Mitra, Saheli, et al.
Publicado: (2023)

A Broadly Neutralizing Antibody Targets the Dynamic HIV Envelope Trimer Apex via a Long, Rigidified, and Anionic β-Hairpin Structure
por: Lee, Jeong Hyun, et al.
Publicado: (2017)

Multiscale analysis of enantioselectivity in enzyme-catalysed ‘lethal synthesis’ using projector-based embedding
por: Zhang, Xinglong, et al.
Publicado: (2018)

De novo active sites for resurrected Precambrian enzymes
por: Risso, Valeria A., et al.
Publicado: (2017)

Enhancing a de novo enzyme activity by computationally-focused ultra-low-throughput screening
por: Risso, Valeria A., et al.
Publicado: (2020)

EPR spectroscopy of whole blood and blood components: can we diagnose abnormalities?
por: Svistunenko, Dimitri A.
Publicado: (2021)

Calculation of Heat Capacity Changes in Enzyme Catalysis and Ligand Binding
por: Åqvist, Johan, et al.
Publicado: (2022)

Weak binding to the A2RE RNA rigidifies hnRNPA2 RRMs and reduces liquid–liquid phase separation and aggregation
por: Ryan, Veronica H, et al.
Publicado: (2020)

Structural resolution of switchable states of a de novo peptide assembly
por: Dawson, William M., et al.
Publicado: (2021)

Reliable In Silico Ranking of Engineered Therapeutic TCR Binding Affinities with MMPB/GBSA
por: Crean, Rory M., et al.
Publicado: (2022)

Tyrosyl radical in haemoglobin and haptoglobin-haemoglobin complex: how does haptoglobin make haemoglobin less toxic?
por: Svistunenko, Dimitri A., et al.
Publicado: (2020)

New insights into controlling radical migration pathways in heme enzymes gained from the study of a dye-decolorising peroxidase
por: Lučić, Marina, et al.
Publicado: (2023)

Natural variants modify Klebsiella pneumoniae carbapenemase (KPC) acyl–enzyme conformational dynamics to extend antibiotic resistance
por: Tooke, Catherine L., et al.
Publicado: (2020)

The effect of heat acclimation or acclimatisation on exercise performance and capacity in the heat: preliminary meta-analysis data
por: Reeve, Tom, et al.
Publicado: (2015)

Synthesis of Rigidified eIF4E/eIF4G Inhibitor-1 (4EGI-1) Mimetic and Their in Vitro Characterization as Inhibitors of Protein–Protein Interaction
por: Mahalingam, Poornachandran, et al.
Publicado: (2014)

Generalized Born Implicit Solvent Models Do Not Reproduce Secondary Structures of De Novo Designed Glu/Lys Peptides
por: Lang, Eric J. M., et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_cthsn3e11llpr5ook8ahaghcr3