Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning

Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be identified for...

Descripción completa

Detalles Bibliográficos
Autores principales: Haas, Cyrus M., Francino-Urdaniz, Irene M., Steiner, Paul J., Whitehead, Timothy A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455247/
https://www.ncbi.nlm.nih.gov/pubmed/34568839
http://dx.doi.org/10.1016/j.xpro.2021.100869

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455247/
https://www.ncbi.nlm.nih.gov/pubmed/34568839
http://dx.doi.org/10.1016/j.xpro.2021.100869

Ejemplares similares