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Identification of SARS-CoV-2 S RBD escape mutants using yeast screening and deep mutational scanning

Here, we describe a protocol to identify escape mutants on the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) spike (S) receptor-binding domain (RBD) using a yeast screen combined with deep mutational scanning. Over 90% of all potential single S RBD escape mutants can be identified for...

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Detalles Bibliográficos
Autores principales:	Haas, Cyrus M., Francino-Urdaniz, Irene M., Steiner, Paul J., Whitehead, Timothy A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455247/ https://www.ncbi.nlm.nih.gov/pubmed/34568839 http://dx.doi.org/10.1016/j.xpro.2021.100869

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