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A Split-Cre system designed to detect simultaneous expression of two genes based on SpyTag/SpyCatcher conjugation and Split-GFP dimerization

The Split-Cre system is a powerful tool for genetic manipulation and can be used to spatiotemporally control gene expression in vivo. However, the low activity of the reconstituted NCre/CCre recombinase in the Split-Cre system limits its application as an indicator of the simultaneous expression of...

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Detalles Bibliográficos
Autores principales: Wei, Xundong, Zhang, Jianhua, Cui, Jian, Xu, Wei, Zhou, Xuyu, Ma, Jie
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society for Biochemistry and Molecular Biology 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455372/
https://www.ncbi.nlm.nih.gov/pubmed/34450162
http://dx.doi.org/10.1016/j.jbc.2021.101119