A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids

Patient-derived organoids (PDOs) recapitulate the cellular heterogeneity of the original colorectal tumor tissue. Here, we describe a protocol to generate genetically modified PDOs to investigate cancer stem cells. This protocol uses the CRISPR-Cas9 system to knock-in the IRES-EGFP-P2A-iCaspase9 cas...

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Detalles Bibliográficos
Autores principales: Okamoto, Takuya, Natsume, Yasuko, Yamanaka, Hitomi, Fukuda, Mayuko, Yao, Ryoji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455475/
https://www.ncbi.nlm.nih.gov/pubmed/34585151
http://dx.doi.org/10.1016/j.xpro.2021.100780

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455475/
https://www.ncbi.nlm.nih.gov/pubmed/34585151
http://dx.doi.org/10.1016/j.xpro.2021.100780

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