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A protocol for efficient CRISPR-Cas9-mediated knock-in in colorectal cancer patient-derived organoids
Patient-derived organoids (PDOs) recapitulate the cellular heterogeneity of the original colorectal tumor tissue. Here, we describe a protocol to generate genetically modified PDOs to investigate cancer stem cells. This protocol uses the CRISPR-Cas9 system to knock-in the IRES-EGFP-P2A-iCaspase9 cas...
Autores principales: | Okamoto, Takuya, Natsume, Yasuko, Yamanaka, Hitomi, Fukuda, Mayuko, Yao, Ryoji |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455475/ https://www.ncbi.nlm.nih.gov/pubmed/34585151 http://dx.doi.org/10.1016/j.xpro.2021.100780 |
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