Nuclei isolation from surgically resected human hippocampus

Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isola...

Descripción completa

Detalles Bibliográficos
Autores principales: Ayhan, Fatma, Douglas, Connor, Lega, Bradley C., Konopka, Genevieve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455478/
https://www.ncbi.nlm.nih.gov/pubmed/34585170
http://dx.doi.org/10.1016/j.xpro.2021.100844

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455478/
https://www.ncbi.nlm.nih.gov/pubmed/34585170
http://dx.doi.org/10.1016/j.xpro.2021.100844

Ejemplares similares