Nuclei isolation from surgically resected human hippocampus

Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isola...

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Autores principales: Ayhan, Fatma, Douglas, Connor, Lega, Bradley C., Konopka, Genevieve
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455478/
https://www.ncbi.nlm.nih.gov/pubmed/34585170
http://dx.doi.org/10.1016/j.xpro.2021.100844
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author Ayhan, Fatma
Douglas, Connor
Lega, Bradley C.
Konopka, Genevieve
author_facet Ayhan, Fatma
Douglas, Connor
Lega, Bradley C.
Konopka, Genevieve
author_sort Ayhan, Fatma
collection PubMed
description Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isolation of high-quality nuclei from surgically resected human brain tissue followed by a sucrose gradient yielding neuronal and non-neuronal nuclei enabling unbiased analysis of various cell types. For complete details on the use and execution of this protocol, please refer to Ayhan et al. (2021).
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spelling pubmed-84554782021-09-27 Nuclei isolation from surgically resected human hippocampus Ayhan, Fatma Douglas, Connor Lega, Bradley C. Konopka, Genevieve STAR Protoc Protocol Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isolation of high-quality nuclei from surgically resected human brain tissue followed by a sucrose gradient yielding neuronal and non-neuronal nuclei enabling unbiased analysis of various cell types. For complete details on the use and execution of this protocol, please refer to Ayhan et al. (2021). Elsevier 2021-09-16 /pmc/articles/PMC8455478/ /pubmed/34585170 http://dx.doi.org/10.1016/j.xpro.2021.100844 Text en © 2021 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Ayhan, Fatma
Douglas, Connor
Lega, Bradley C.
Konopka, Genevieve
Nuclei isolation from surgically resected human hippocampus
title Nuclei isolation from surgically resected human hippocampus
title_full Nuclei isolation from surgically resected human hippocampus
title_fullStr Nuclei isolation from surgically resected human hippocampus
title_full_unstemmed Nuclei isolation from surgically resected human hippocampus
title_short Nuclei isolation from surgically resected human hippocampus
title_sort nuclei isolation from surgically resected human hippocampus
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455478/
https://www.ncbi.nlm.nih.gov/pubmed/34585170
http://dx.doi.org/10.1016/j.xpro.2021.100844
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