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Nuclei isolation from surgically resected human hippocampus

Single-nucleus RNA sequencing (snRNA-seq), where nuclear transcriptomes are a proxy to cellular transcriptomes, has been used to profile human brain. snRNA-seq is sensitive to tissue processing, tissue quality, postmortem interval time, and cellular debris. This protocol outlines steps for the isola...

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Detalles Bibliográficos
Autores principales:	Ayhan, Fatma, Douglas, Connor, Lega, Bradley C., Konopka, Genevieve
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2021
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455478/ https://www.ncbi.nlm.nih.gov/pubmed/34585170 http://dx.doi.org/10.1016/j.xpro.2021.100844

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