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Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells
This protocol is intended as a guide for implementing or refining the usage of the air-liquid interface (ALI) model system to generate airway mucociliary tissue in vitro. We present a streamlined protocol for isolating the stem cells from inferior nasal turbinates of donors, allowing for a simple an...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455484/ https://www.ncbi.nlm.nih.gov/pubmed/34585152 http://dx.doi.org/10.1016/j.xpro.2021.100782 |