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Isolation, expansion, differentiation, and histological processing of human nasal epithelial cells

This protocol is intended as a guide for implementing or refining the usage of the air-liquid interface (ALI) model system to generate airway mucociliary tissue in vitro. We present a streamlined protocol for isolating the stem cells from inferior nasal turbinates of donors, allowing for a simple an...

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Detalles Bibliográficos
Autores principales: Manna, Vincent, Caradonna, Salvatore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8455484/
https://www.ncbi.nlm.nih.gov/pubmed/34585152
http://dx.doi.org/10.1016/j.xpro.2021.100782