Screening of CRISPR-Cas9-generated point mutant mice using MiSeq and locked nucleic acid probe PCR

CRISPR-Cas9-mediated, site-directed mutagenesis in mice generates mosaic founder mice with varied efficiency of desired point mutation and other non-homologous end-joined variants. Here, we present a protocol for design, sample preparation, and analysis for identification of mice with the desired mu...

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Detalles Bibliográficos
Autores principales: Vasu, Kommireddy, Fox, Paul L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456059/
https://www.ncbi.nlm.nih.gov/pubmed/34585153
http://dx.doi.org/10.1016/j.xpro.2021.100785

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456059/
https://www.ncbi.nlm.nih.gov/pubmed/34585153
http://dx.doi.org/10.1016/j.xpro.2021.100785

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