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Screening of CRISPR-Cas9-generated point mutant mice using MiSeq and locked nucleic acid probe PCR
CRISPR-Cas9-mediated, site-directed mutagenesis in mice generates mosaic founder mice with varied efficiency of desired point mutation and other non-homologous end-joined variants. Here, we present a protocol for design, sample preparation, and analysis for identification of mice with the desired mu...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456059/ https://www.ncbi.nlm.nih.gov/pubmed/34585153 http://dx.doi.org/10.1016/j.xpro.2021.100785 |