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Genome editing in archaeal viruses and endogenous viral protein purification

Archaea-infecting viruses are morphologically and genomically among the most diverse entities. Unfortunately, they are also fairly understudied due to a lack of efficient genetic tools. Here, we present a detailed protocol for the CRISPR/Cas-based genome editing of the virus SIRV2 infecting the genu...

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Detalles Bibliográficos
Autores principales: Alfastsen, Lauge, Peng, Xu, Bhoobalan-Chitty, Yuvaraj
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456065/
https://www.ncbi.nlm.nih.gov/pubmed/34585154
http://dx.doi.org/10.1016/j.xpro.2021.100791