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Genome editing in archaeal viruses and endogenous viral protein purification
Archaea-infecting viruses are morphologically and genomically among the most diverse entities. Unfortunately, they are also fairly understudied due to a lack of efficient genetic tools. Here, we present a detailed protocol for the CRISPR/Cas-based genome editing of the virus SIRV2 infecting the genu...
Autores principales: | Alfastsen, Lauge, Peng, Xu, Bhoobalan-Chitty, Yuvaraj |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456065/ https://www.ncbi.nlm.nih.gov/pubmed/34585154 http://dx.doi.org/10.1016/j.xpro.2021.100791 |
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