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Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer
Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456108/ https://www.ncbi.nlm.nih.gov/pubmed/34585168 http://dx.doi.org/10.1016/j.xpro.2021.100841 |