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Tumor dissociation of highly viable cell suspensions for single-cell omic analyses in mouse models of breast cancer

Cell preparation with a high rate of viable cells is required to obtain reliable single-cell transcriptomic and epigenomic data. This protocol describes a technique for digestion and single-cell isolation from mouse mammary tumors to achieve ∼90% of viable cells, which can be subsequently processed...

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Detalles Bibliográficos
Autores principales: Rodriguez de la Fuente, Laura, Law, Andrew M.K., Gallego-Ortega, David, Valdes-Mora, Fatima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8456108/
https://www.ncbi.nlm.nih.gov/pubmed/34585168
http://dx.doi.org/10.1016/j.xpro.2021.100841