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Evaluation of extraction-free RT-PCR methods for faster and cheaper detection of SARS-CoV-2 using two commercial systems

OBJECTIVE: When using high-throughput batched diagnostic platforms based on RT-PCR for SARS-CoV-2 detection, avoidance of the conventional nucleic acid extraction step can help to reduce the turnaround time and increase processivity. This approach can also spare reagents and plasticware, which have...

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Detalles Bibliográficos
Autores principales: Morecchiato, Fabio, Coppi, Marco, Baccani, Ilaria, Maggini, Niccolò, Ciccone, Nunziata, Antonelli, Alberto, Rossolini, Gian Maria
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. Published by Elsevier Ltd on behalf of International Society for Infectious Diseases. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8458106/
https://www.ncbi.nlm.nih.gov/pubmed/34563710
http://dx.doi.org/10.1016/j.ijid.2021.09.046