Generation of a more efficient prime editor 2 by addition of the Rad51 DNA-binding domain

Although prime editing is a promising genome editing method, the efficiency of prime editor 2 (PE2) is often insufficient. Here we generate a more efficient variant of PE2, named hyPE2, by adding the Rad51 DNA-binding domain. When tested at endogenous sites, hyPE2 shows a median of 1.5- or 1.4- fold...

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Detalles Bibliográficos
Autores principales: Song, Myungjae, Lim, Jung Min, Min, Seonwoo, Oh, Jeong-Seok, Kim, Dong Young, Woo, Jae-Sung, Nishimasu, Hiroshi, Cho, Sung-Rae, Yoon, Sungroh, Kim, Hyongbum Henry
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460726/
https://www.ncbi.nlm.nih.gov/pubmed/34556671
http://dx.doi.org/10.1038/s41467-021-25928-2

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8460726/
https://www.ncbi.nlm.nih.gov/pubmed/34556671
http://dx.doi.org/10.1038/s41467-021-25928-2

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