Rapid generation of conditional knockout mice using the CRISPR-Cas9 system and electroporation for neuroscience research

The Cre/LoxP-based conditional knockout technology is a powerful tool for gene function analysis that allows region- and time-specific gene manipulation. However, inserting a pair of LoxP cassettes to generate conditional knockout can be technically challenging and thus time- and resource-consuming....

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Detalles Bibliográficos
Autores principales: Nishizono, Hirofumi, Hayano, Yuki, Nakahata, Yoshihisa, Ishigaki, Yasuhito, Yasuda, Ryohei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Micro Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461926/
https://www.ncbi.nlm.nih.gov/pubmed/34556164
http://dx.doi.org/10.1186/s13041-021-00859-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8461926/
https://www.ncbi.nlm.nih.gov/pubmed/34556164
http://dx.doi.org/10.1186/s13041-021-00859-7

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