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Improving stool sample processing and pyrosequencing for quantifying benzimidazole resistance alleles in Trichuris trichiura and Necator americanus pooled eggs

BACKGROUND: There is an urgent need for an extensive evaluation of benzimidazole efficacy in humans. In veterinary science, benzimidazole resistance has been mainly associated with three single-nucleotide polymorphisms (SNPs) in the isotype-1 β-tubulin gene. In this study, we optimized the stool sam...

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Detalles Bibliográficos
Autores principales: Gandasegui, Javier, Grau-Pujol, Berta, Cambra-Pelleja, María, Escola, Valdemiro, Demontis, Maria Antonietta, Cossa, Anelsio, Jamine, José Carlos, Balaña-Fouce, Rafael, van Lieshout, Lisette, Muñoz, José, Martínez-Valladares, María
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8466976/
https://www.ncbi.nlm.nih.gov/pubmed/34563247
http://dx.doi.org/10.1186/s13071-021-04941-w