Analyzing Plant Gene Targeting Outcomes and Conversion Tracts with Nanopore Sequencing

The high-throughput molecular analysis of gene targeting (GT) events is made technically challenging by the residual presetabce of donor molecules. Large donor molecules restrict primer placement, resulting in long amplicons that cannot be readily analyzed using standard NGS pipelines or qPCR-based...

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Detalles Bibliográficos
Autores principales: Atkins, Paul A. P., Gamo, Maria Elena S., Voytas, Daniel F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467259/
https://www.ncbi.nlm.nih.gov/pubmed/34575882
http://dx.doi.org/10.3390/ijms22189723

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467259/
https://www.ncbi.nlm.nih.gov/pubmed/34575882
http://dx.doi.org/10.3390/ijms22189723

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