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Analyzing Plant Gene Targeting Outcomes and Conversion Tracts with Nanopore Sequencing
The high-throughput molecular analysis of gene targeting (GT) events is made technically challenging by the residual presetabce of donor molecules. Large donor molecules restrict primer placement, resulting in long amplicons that cannot be readily analyzed using standard NGS pipelines or qPCR-based...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
MDPI
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8467259/ https://www.ncbi.nlm.nih.gov/pubmed/34575882 http://dx.doi.org/10.3390/ijms22189723 |