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A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides

In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris expression vectors. Twenty E. coli and ten P. pas...

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Detalles Bibliográficos
Autores principales: Zhang, Yingli, Li, Zhongchen, Li, Li, Rao, Ben, Ma, Lixin, Wang, Yaping
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469748/
https://www.ncbi.nlm.nih.gov/pubmed/34576753
http://dx.doi.org/10.3390/microorganisms9091858