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A Method for Rapid Screening, Expression, and Purification of Antimicrobial Peptides
In this study, a method for the rapid screening, expression and purification of antimicrobial peptides (AMPs) was developed. AMP genes were fused to a heat-resistant CL7 tag using the SLOPE method, and cloned into Escherichia coli and Pichia pastoris expression vectors. Twenty E. coli and ten P. pas...
Autores principales: | Zhang, Yingli, Li, Zhongchen, Li, Li, Rao, Ben, Ma, Lixin, Wang, Yaping |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8469748/ https://www.ncbi.nlm.nih.gov/pubmed/34576753 http://dx.doi.org/10.3390/microorganisms9091858 |
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