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Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea

Vibriosis in farmed animals is a serious threat to aquaculture worldwide. Using probiotics and anti-Vibrio antimicrobial substances in aquaculture systems can be a means of preventing Vibrio infections. Therefore, we aimed to characterize and compare 16 potential anti-Vibrio probiotics (Vi+) isolate...

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Autores principales: Chau, Khanh Minh, Van, Thi Thu Hao, Quyen, Dong Van, Le, Hung Dinh, Phan, Trinh Hoai Thi, Ngo, Ngoc Duy Thi, Vo, Trang Dieu Thi, Dinh, Trung Thanh, Le, Hoa Thi, Khanh, Huynh Hoang Nhu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470590/
https://www.ncbi.nlm.nih.gov/pubmed/34576821
http://dx.doi.org/10.3390/microorganisms9091927
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author Chau, Khanh Minh
Van, Thi Thu Hao
Quyen, Dong Van
Le, Hung Dinh
Phan, Trinh Hoai Thi
Ngo, Ngoc Duy Thi
Vo, Trang Dieu Thi
Dinh, Trung Thanh
Le, Hoa Thi
Khanh, Huynh Hoang Nhu
author_facet Chau, Khanh Minh
Van, Thi Thu Hao
Quyen, Dong Van
Le, Hung Dinh
Phan, Trinh Hoai Thi
Ngo, Ngoc Duy Thi
Vo, Trang Dieu Thi
Dinh, Trung Thanh
Le, Hoa Thi
Khanh, Huynh Hoang Nhu
author_sort Chau, Khanh Minh
collection PubMed
description Vibriosis in farmed animals is a serious threat to aquaculture worldwide. Using probiotics and anti-Vibrio antimicrobial substances in aquaculture systems can be a means of preventing Vibrio infections. Therefore, we aimed to characterize and compare 16 potential anti-Vibrio probiotics (Vi+) isolated from marine sponges and fish intestines collected from the Vietnam Sea, as well as an anti-Vibrio bacteriocin to fully explore their application potentials. 16S rRNA sequencing confirmed all Vi+ to be Bacillus species with different strain variants across two sample types. An obvious antimicrobial spectrum toward Gram-negative bacteria was observed from intestinal Vi+ compared to sponge-associated Vi+. The reason was the higher gene frequency of two antimicrobial compounds, non-ribosomal peptides (NRPS) and polyketide type-I (PKS-I) from intestinal Vi+ (66.7%) than sponge-associated Vi+ (14.3% and 0%, respectively). Additionally, a three-step procedure was performed to purify an anti-Vibrio bacteriocin produced by B. methylotrophicus NTBD1, including (i) solvent extraction of bacteriocin from cells, (ii) hydrophobic interaction chromatography, and (iii) reverse-phase HPLC. The bacteriocin had a molecular weight of ~2–5 kDa, was sensitive to proteolysis and thermally stable, and showed a broad antimicrobial spectrum, all of which are essential properties for promising feed additives. This study provides necessary information of the potential of probiotic Bacillus species with anti-Vibrio antimicrobial properties to study their further use in sustainable aquaculture.
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spelling pubmed-84705902021-09-27 Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea Chau, Khanh Minh Van, Thi Thu Hao Quyen, Dong Van Le, Hung Dinh Phan, Trinh Hoai Thi Ngo, Ngoc Duy Thi Vo, Trang Dieu Thi Dinh, Trung Thanh Le, Hoa Thi Khanh, Huynh Hoang Nhu Microorganisms Article Vibriosis in farmed animals is a serious threat to aquaculture worldwide. Using probiotics and anti-Vibrio antimicrobial substances in aquaculture systems can be a means of preventing Vibrio infections. Therefore, we aimed to characterize and compare 16 potential anti-Vibrio probiotics (Vi+) isolated from marine sponges and fish intestines collected from the Vietnam Sea, as well as an anti-Vibrio bacteriocin to fully explore their application potentials. 16S rRNA sequencing confirmed all Vi+ to be Bacillus species with different strain variants across two sample types. An obvious antimicrobial spectrum toward Gram-negative bacteria was observed from intestinal Vi+ compared to sponge-associated Vi+. The reason was the higher gene frequency of two antimicrobial compounds, non-ribosomal peptides (NRPS) and polyketide type-I (PKS-I) from intestinal Vi+ (66.7%) than sponge-associated Vi+ (14.3% and 0%, respectively). Additionally, a three-step procedure was performed to purify an anti-Vibrio bacteriocin produced by B. methylotrophicus NTBD1, including (i) solvent extraction of bacteriocin from cells, (ii) hydrophobic interaction chromatography, and (iii) reverse-phase HPLC. The bacteriocin had a molecular weight of ~2–5 kDa, was sensitive to proteolysis and thermally stable, and showed a broad antimicrobial spectrum, all of which are essential properties for promising feed additives. This study provides necessary information of the potential of probiotic Bacillus species with anti-Vibrio antimicrobial properties to study their further use in sustainable aquaculture. MDPI 2021-09-10 /pmc/articles/PMC8470590/ /pubmed/34576821 http://dx.doi.org/10.3390/microorganisms9091927 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Chau, Khanh Minh
Van, Thi Thu Hao
Quyen, Dong Van
Le, Hung Dinh
Phan, Trinh Hoai Thi
Ngo, Ngoc Duy Thi
Vo, Trang Dieu Thi
Dinh, Trung Thanh
Le, Hoa Thi
Khanh, Huynh Hoang Nhu
Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea
title Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea
title_full Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea
title_fullStr Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea
title_full_unstemmed Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea
title_short Molecular Identification and Characterization of Probiotic Bacillus Species with the Ability to Control Vibrio spp. in Wild Fish Intestines and Sponges from the Vietnam Sea
title_sort molecular identification and characterization of probiotic bacillus species with the ability to control vibrio spp. in wild fish intestines and sponges from the vietnam sea
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8470590/
https://www.ncbi.nlm.nih.gov/pubmed/34576821
http://dx.doi.org/10.3390/microorganisms9091927
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