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Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was st...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471563/ https://www.ncbi.nlm.nih.gov/pubmed/34578703 http://dx.doi.org/10.3390/nano11092387 |
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author | Klein, Anna Lena Lubda, Markus Specht, David Pyo, Sung-Min Busch, Loris Lademann, Jürgen Meinke, Martina C. Beckers, Ingeborg von Hagen, Jörg Keck, Cornelia M. Patzelt, Alexa |
author_facet | Klein, Anna Lena Lubda, Markus Specht, David Pyo, Sung-Min Busch, Loris Lademann, Jürgen Meinke, Martina C. Beckers, Ingeborg von Hagen, Jörg Keck, Cornelia M. Patzelt, Alexa |
author_sort | Klein, Anna Lena |
collection | PubMed |
description | Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was studied. In setup A, a caffeine nanocrystal formulation was compared to a non-particular caffeine gel formulation. In setup B, two caffeine nanocrystal formulations of different sizes (200 nm, 700 nm) were compared to each other. Microdialysis samples were collected for 46 h. After sampling, the skin layers were separated, homogenized, and caffeine was quantified in all samples. In setup A the area under the curve (AUC) after crystal gel formulation application was 12 times higher than after non-particular formulation application. Setup B showed an increased AUC of 42% in the microdialysis data when the 700 nm caffeine crystals were applied compared to the 200 nm crystals. The microdialysis data was supported by the separation, homogenization and extraction data. Microdialysis performed on ex vivo porcine ear skin is a novel experimental setup. It is of high interest for further investigations since it is able to also capture the impact of follicular and transfollicular penetration kinetics as no other ex vivo setup can. |
format | Online Article Text |
id | pubmed-8471563 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-84715632021-09-28 Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals Klein, Anna Lena Lubda, Markus Specht, David Pyo, Sung-Min Busch, Loris Lademann, Jürgen Meinke, Martina C. Beckers, Ingeborg von Hagen, Jörg Keck, Cornelia M. Patzelt, Alexa Nanomaterials (Basel) Article Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was studied. In setup A, a caffeine nanocrystal formulation was compared to a non-particular caffeine gel formulation. In setup B, two caffeine nanocrystal formulations of different sizes (200 nm, 700 nm) were compared to each other. Microdialysis samples were collected for 46 h. After sampling, the skin layers were separated, homogenized, and caffeine was quantified in all samples. In setup A the area under the curve (AUC) after crystal gel formulation application was 12 times higher than after non-particular formulation application. Setup B showed an increased AUC of 42% in the microdialysis data when the 700 nm caffeine crystals were applied compared to the 200 nm crystals. The microdialysis data was supported by the separation, homogenization and extraction data. Microdialysis performed on ex vivo porcine ear skin is a novel experimental setup. It is of high interest for further investigations since it is able to also capture the impact of follicular and transfollicular penetration kinetics as no other ex vivo setup can. MDPI 2021-09-14 /pmc/articles/PMC8471563/ /pubmed/34578703 http://dx.doi.org/10.3390/nano11092387 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Klein, Anna Lena Lubda, Markus Specht, David Pyo, Sung-Min Busch, Loris Lademann, Jürgen Meinke, Martina C. Beckers, Ingeborg von Hagen, Jörg Keck, Cornelia M. Patzelt, Alexa Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals |
title | Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals |
title_full | Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals |
title_fullStr | Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals |
title_full_unstemmed | Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals |
title_short | Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals |
title_sort | microdialysis on ex vivo porcine ear skin can validly study dermal penetration including the fraction of transfollicular penetration—demonstrated on caffeine nanocrystals |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471563/ https://www.ncbi.nlm.nih.gov/pubmed/34578703 http://dx.doi.org/10.3390/nano11092387 |
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