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Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals

Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was st...

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Autores principales: Klein, Anna Lena, Lubda, Markus, Specht, David, Pyo, Sung-Min, Busch, Loris, Lademann, Jürgen, Meinke, Martina C., Beckers, Ingeborg, von Hagen, Jörg, Keck, Cornelia M., Patzelt, Alexa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471563/
https://www.ncbi.nlm.nih.gov/pubmed/34578703
http://dx.doi.org/10.3390/nano11092387
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author Klein, Anna Lena
Lubda, Markus
Specht, David
Pyo, Sung-Min
Busch, Loris
Lademann, Jürgen
Meinke, Martina C.
Beckers, Ingeborg
von Hagen, Jörg
Keck, Cornelia M.
Patzelt, Alexa
author_facet Klein, Anna Lena
Lubda, Markus
Specht, David
Pyo, Sung-Min
Busch, Loris
Lademann, Jürgen
Meinke, Martina C.
Beckers, Ingeborg
von Hagen, Jörg
Keck, Cornelia M.
Patzelt, Alexa
author_sort Klein, Anna Lena
collection PubMed
description Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was studied. In setup A, a caffeine nanocrystal formulation was compared to a non-particular caffeine gel formulation. In setup B, two caffeine nanocrystal formulations of different sizes (200 nm, 700 nm) were compared to each other. Microdialysis samples were collected for 46 h. After sampling, the skin layers were separated, homogenized, and caffeine was quantified in all samples. In setup A the area under the curve (AUC) after crystal gel formulation application was 12 times higher than after non-particular formulation application. Setup B showed an increased AUC of 42% in the microdialysis data when the 700 nm caffeine crystals were applied compared to the 200 nm crystals. The microdialysis data was supported by the separation, homogenization and extraction data. Microdialysis performed on ex vivo porcine ear skin is a novel experimental setup. It is of high interest for further investigations since it is able to also capture the impact of follicular and transfollicular penetration kinetics as no other ex vivo setup can.
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spelling pubmed-84715632021-09-28 Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals Klein, Anna Lena Lubda, Markus Specht, David Pyo, Sung-Min Busch, Loris Lademann, Jürgen Meinke, Martina C. Beckers, Ingeborg von Hagen, Jörg Keck, Cornelia M. Patzelt, Alexa Nanomaterials (Basel) Article Common ex vivo methods for penetration investigations often fail to monitor transfollicular penetration appropriately. In the present investigation, the validity of dermal microdialysis on the ex vivo porcine ear skin to investigate penetration kinetics, including transfollicular penetration, was studied. In setup A, a caffeine nanocrystal formulation was compared to a non-particular caffeine gel formulation. In setup B, two caffeine nanocrystal formulations of different sizes (200 nm, 700 nm) were compared to each other. Microdialysis samples were collected for 46 h. After sampling, the skin layers were separated, homogenized, and caffeine was quantified in all samples. In setup A the area under the curve (AUC) after crystal gel formulation application was 12 times higher than after non-particular formulation application. Setup B showed an increased AUC of 42% in the microdialysis data when the 700 nm caffeine crystals were applied compared to the 200 nm crystals. The microdialysis data was supported by the separation, homogenization and extraction data. Microdialysis performed on ex vivo porcine ear skin is a novel experimental setup. It is of high interest for further investigations since it is able to also capture the impact of follicular and transfollicular penetration kinetics as no other ex vivo setup can. MDPI 2021-09-14 /pmc/articles/PMC8471563/ /pubmed/34578703 http://dx.doi.org/10.3390/nano11092387 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Klein, Anna Lena
Lubda, Markus
Specht, David
Pyo, Sung-Min
Busch, Loris
Lademann, Jürgen
Meinke, Martina C.
Beckers, Ingeborg
von Hagen, Jörg
Keck, Cornelia M.
Patzelt, Alexa
Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
title Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
title_full Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
title_fullStr Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
title_full_unstemmed Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
title_short Microdialysis on Ex Vivo Porcine Ear Skin Can Validly Study Dermal Penetration including the Fraction of Transfollicular Penetration—Demonstrated on Caffeine Nanocrystals
title_sort microdialysis on ex vivo porcine ear skin can validly study dermal penetration including the fraction of transfollicular penetration—demonstrated on caffeine nanocrystals
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8471563/
https://www.ncbi.nlm.nih.gov/pubmed/34578703
http://dx.doi.org/10.3390/nano11092387
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