Cargando…

Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland

Within Europe over the last 10 years, there has been an increase in seaweeds cultivated for human consumption. For food safety reasons, it is important to assess the microbiological and nutritional quality of the biomass. The fresh and dried edible seaweeds Alaria esculenta and Saccharina latissima...

Descripción completa

Detalles Bibliográficos
Autores principales: Lytou, Anastasia E., Schoina, Eirini, Liu, Yunge, Michalek, Kati, Stanley, Michele S., Panagou, Efstathios Z., Nychas, George-John E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472205/
https://www.ncbi.nlm.nih.gov/pubmed/34574321
http://dx.doi.org/10.3390/foods10092210
_version_ 1784574669026754560
author Lytou, Anastasia E.
Schoina, Eirini
Liu, Yunge
Michalek, Kati
Stanley, Michele S.
Panagou, Efstathios Z.
Nychas, George-John E.
author_facet Lytou, Anastasia E.
Schoina, Eirini
Liu, Yunge
Michalek, Kati
Stanley, Michele S.
Panagou, Efstathios Z.
Nychas, George-John E.
author_sort Lytou, Anastasia E.
collection PubMed
description Within Europe over the last 10 years, there has been an increase in seaweeds cultivated for human consumption. For food safety reasons, it is important to assess the microbiological and nutritional quality of the biomass. The fresh and dried edible seaweeds Alaria esculenta and Saccharina latissima were assessed over two consecutive years for the presence of microorganisms. Seaweed samples supplied from Scotland were stored under isothermal conditions for specific time intervals depending on the sample’s condition (fresh, dried or rehydrated). During storage, microbiological analyses were performed for the enumeration of Total Viable Counts (TVC), Pseudomonas spp., Enterobacteriaceae and Bacillus spp., as well as yeasts and molds. Additionally, bacterial colonies from the Marine Agar growth medium were isolated and subjected to PCR-RAPD analysis for characterization of the bacterial diversity of seaweeds. Bacterial isolates with different fingerprint patterns were further subjected to sequencing (16S rDNA, V1–V4 region). The presence of human pathogenic bacteria was also investigated. Results showed that the initial population of TVC was differentiated depending on the year of seaweed harvest, being closer to the enumeration limit (1.0 log CFU/g) in fresh samples from 2020 and higher in samples from 2019 (6.7 and 3.9 log CFU/g in A. esculenta and S. latissima, respectively). DNA-based analysis revealed the presence of Psychrobacter, Cobetia and Pseudomonas species in A. esculenta, while Psychrobacter and Micrococcus species were present in S. latissima.
format Online
Article
Text
id pubmed-8472205
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-84722052021-09-28 Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland Lytou, Anastasia E. Schoina, Eirini Liu, Yunge Michalek, Kati Stanley, Michele S. Panagou, Efstathios Z. Nychas, George-John E. Foods Article Within Europe over the last 10 years, there has been an increase in seaweeds cultivated for human consumption. For food safety reasons, it is important to assess the microbiological and nutritional quality of the biomass. The fresh and dried edible seaweeds Alaria esculenta and Saccharina latissima were assessed over two consecutive years for the presence of microorganisms. Seaweed samples supplied from Scotland were stored under isothermal conditions for specific time intervals depending on the sample’s condition (fresh, dried or rehydrated). During storage, microbiological analyses were performed for the enumeration of Total Viable Counts (TVC), Pseudomonas spp., Enterobacteriaceae and Bacillus spp., as well as yeasts and molds. Additionally, bacterial colonies from the Marine Agar growth medium were isolated and subjected to PCR-RAPD analysis for characterization of the bacterial diversity of seaweeds. Bacterial isolates with different fingerprint patterns were further subjected to sequencing (16S rDNA, V1–V4 region). The presence of human pathogenic bacteria was also investigated. Results showed that the initial population of TVC was differentiated depending on the year of seaweed harvest, being closer to the enumeration limit (1.0 log CFU/g) in fresh samples from 2020 and higher in samples from 2019 (6.7 and 3.9 log CFU/g in A. esculenta and S. latissima, respectively). DNA-based analysis revealed the presence of Psychrobacter, Cobetia and Pseudomonas species in A. esculenta, while Psychrobacter and Micrococcus species were present in S. latissima. MDPI 2021-09-17 /pmc/articles/PMC8472205/ /pubmed/34574321 http://dx.doi.org/10.3390/foods10092210 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lytou, Anastasia E.
Schoina, Eirini
Liu, Yunge
Michalek, Kati
Stanley, Michele S.
Panagou, Efstathios Z.
Nychas, George-John E.
Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland
title Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland
title_full Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland
title_fullStr Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland
title_full_unstemmed Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland
title_short Quality and Safety Assessment of Edible Seaweeds Alaria esculenta and Saccharina latissima Cultivated in Scotland
title_sort quality and safety assessment of edible seaweeds alaria esculenta and saccharina latissima cultivated in scotland
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472205/
https://www.ncbi.nlm.nih.gov/pubmed/34574321
http://dx.doi.org/10.3390/foods10092210
work_keys_str_mv AT lytouanastasiae qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland
AT schoinaeirini qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland
AT liuyunge qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland
AT michalekkati qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland
AT stanleymicheles qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland
AT panagouefstathiosz qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland
AT nychasgeorgejohne qualityandsafetyassessmentofedibleseaweedsalariaesculentaandsaccharinalatissimacultivatedinscotland