Regulating the T7 RNA polymerase expression in E. coli BL21 (DE3) to provide more host options for recombinant protein production

Escherichia coli is the most widely used bacterium in prokaryotic expression system for the production of recombinant proteins. In BL21 (DE3), the gene encoding the T7 RNA polymerase (T7 RNAP) is under control of the strong lacUV5 promoter (P(lacUV5)), which is leakier and more active than wild-type...

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Detalles Bibliográficos
Autores principales: Du, Fei, Liu, Yun-Qi, Xu, Ying-Shuang, Li, Zi-Jia, Wang, Yu-Zhou, Zhang, Zi-Xu, Sun, Xiao-Man
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474846/
https://www.ncbi.nlm.nih.gov/pubmed/34565359
http://dx.doi.org/10.1186/s12934-021-01680-6

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474846/
https://www.ncbi.nlm.nih.gov/pubmed/34565359
http://dx.doi.org/10.1186/s12934-021-01680-6

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