Improved library preparation protocols for amplicon sequencing-based noninvasive fetal genotyping for RHD-positive D antigen-negative alleles

OBJECTIVE: We aimed to simplify our fetal RHD genotyping protocol by changing the method to attach Illumina’s sequencing adaptors to PCR products from the ligation-based method to a PCR-based method, and to improve its reliability and robustness by introducing unique molecular indexes, which allow u...

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Detalles Bibliográficos
Autores principales: Hori, Asuka, Ogata-Kawata, Hiroko, Sasaki, Aiko, Takahashi, Ken, Taniguchi, Kosuke, Migita, Ohsuke, Kawashima, Akihiro, Okamoto, Aikou, Sekizawa, Akihiko, Sago, Haruhiko, Takada, Fumio, Nakabayashi, Kazuhiko, Hata, Kenichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Research Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474863/
https://www.ncbi.nlm.nih.gov/pubmed/34565457
http://dx.doi.org/10.1186/s13104-021-05793-4

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8474863/
https://www.ncbi.nlm.nih.gov/pubmed/34565457
http://dx.doi.org/10.1186/s13104-021-05793-4

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