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Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus

As an environmentally friendly and efficient method, successive two-step fermentation has been applied for extracting chitin from shrimp shells. To screen out the microorganisms for fermentation, a protease-producing strain, Exiguobacterium profundum, and a lactic acid-producing strain, Lactobacillu...

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Autores principales: Xie, Jingwen, Xie, Wancui, Yu, Jing, Xin, Rongyu, Shi, Zhenping, Song, Lin, Yang, Xihong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476949/
https://www.ncbi.nlm.nih.gov/pubmed/34594309
http://dx.doi.org/10.3389/fmicb.2021.677126
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author Xie, Jingwen
Xie, Wancui
Yu, Jing
Xin, Rongyu
Shi, Zhenping
Song, Lin
Yang, Xihong
author_facet Xie, Jingwen
Xie, Wancui
Yu, Jing
Xin, Rongyu
Shi, Zhenping
Song, Lin
Yang, Xihong
author_sort Xie, Jingwen
collection PubMed
description As an environmentally friendly and efficient method, successive two-step fermentation has been applied for extracting chitin from shrimp shells. To screen out the microorganisms for fermentation, a protease-producing strain, Exiguobacterium profundum, and a lactic acid-producing strain, Lactobacillus acidophilus, were isolated from the traditional fermented shrimp paste. Chitin was extracted by successive two-step fermentation with these two strains, and 85.9 ± 1.2% of protein and 95 ± 3% of minerals were removed. The recovery and yield of chitin were 47.82 and 16.32%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM) were used to characterize the chitin. The crystallinity index was 54.37%, and the degree of deacetylation was 3.67%, which was lower than that of chitin extracted by the chemical method. These results indicated that successive two-step fermentation using these two bacterial strains could be applied to extract chitin. This work provides a suitable strategy for developing an effective method to extract chitin by microbial fermentation.
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spelling pubmed-84769492021-09-29 Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus Xie, Jingwen Xie, Wancui Yu, Jing Xin, Rongyu Shi, Zhenping Song, Lin Yang, Xihong Front Microbiol Microbiology As an environmentally friendly and efficient method, successive two-step fermentation has been applied for extracting chitin from shrimp shells. To screen out the microorganisms for fermentation, a protease-producing strain, Exiguobacterium profundum, and a lactic acid-producing strain, Lactobacillus acidophilus, were isolated from the traditional fermented shrimp paste. Chitin was extracted by successive two-step fermentation with these two strains, and 85.9 ± 1.2% of protein and 95 ± 3% of minerals were removed. The recovery and yield of chitin were 47.82 and 16.32%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM) were used to characterize the chitin. The crystallinity index was 54.37%, and the degree of deacetylation was 3.67%, which was lower than that of chitin extracted by the chemical method. These results indicated that successive two-step fermentation using these two bacterial strains could be applied to extract chitin. This work provides a suitable strategy for developing an effective method to extract chitin by microbial fermentation. Frontiers Media S.A. 2021-09-14 /pmc/articles/PMC8476949/ /pubmed/34594309 http://dx.doi.org/10.3389/fmicb.2021.677126 Text en Copyright © 2021 Xie, Xie, Yu, Xin, Shi, Song and Yang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Xie, Jingwen
Xie, Wancui
Yu, Jing
Xin, Rongyu
Shi, Zhenping
Song, Lin
Yang, Xihong
Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus
title Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus
title_full Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus
title_fullStr Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus
title_full_unstemmed Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus
title_short Extraction of Chitin From Shrimp Shell by Successive Two-Step Fermentation of Exiguobacterium profundum and Lactobacillus acidophilus
title_sort extraction of chitin from shrimp shell by successive two-step fermentation of exiguobacterium profundum and lactobacillus acidophilus
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8476949/
https://www.ncbi.nlm.nih.gov/pubmed/34594309
http://dx.doi.org/10.3389/fmicb.2021.677126
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